Mouse Anti-HSPA8 mAb (Unconjugated, IgG2b, Proclin 300 based) [REK5-K332]
Type: Monoclonal Antibody
1. Summary of Results:
The antibody used: HSPA8 (at 1:2000 dilution)
Secondary antibody: Goat polyclonal to mouse IgG (at 1/50,000 dilution)
Predicted and observed band size: approximately 70-75 kDa
Exposure time: 10 seconds
2.Samples tested:
Whole cell lysates from human cell lines: HeLa, NIH/3T3, K562, HepG2
Tissue samples: Mouse spleen, Rat spleen, Mouse heart, Rat heart
3. Interpretation:
The consistent detection of bands around 70-75 kDa across all samples suggests successful recognition of HSPA8, which is known to be a constitutive and highly expressed heat shock protein in various cell types and tissues.
The band size matches the predicted size, indicating specific detection with minimal non-specific bands.
The short exposure time (10s) suggests a clear, strong signal without oversaturation.
| Key Details | |
|---|---|
| Type | Monoclonal Antibody |
| Target | HSPA8 |
| Species Reactivity | Human;Rat;Mouse |
| Application | ELISA;WB;IHC;Flow Cyt;IP |
| Conjugation/Format | Unconjugated |
| Host | Mouse |
| Immunogen | Recombinant Human Heat Shock Cognate Protein 71 kDa (aa 2-646) |
| Isotype | IgG2b |
| Properties | |
|---|---|
| Form | Liquid |
| Concentration | Differences may occur among batches. Contact us to verify. |
| Dilutions | WB: 1:10000-1:256000, IHC: 1:100-1:500, Flow Cyt: 1:100-1:300, IP: 1μl-4μl. |
| Purification | Purified to>95% purity using Protein A. |
| Storage Buffer | 0.01M PBS+50% Glycerol+0.03% Proclin 300, pH 7.4. |
| Storage/Stability | Store at -20°C or -80°C once received. Avoid freezing and thawing repeatedly. |
| Shipping | Ice Pack |
| Target Aliases | HSPA 8;HSPA-8;HSC70;HSP73;HSPA8_HUMAN;Heat shock cognate 71 kDa protein;Constitutive heat shock protein 70 |
