Mouse Anti-KRT13 mAb (Unconjugated) [REK5-K369]

IHC was performed on paraffin-embedded human tonsil tissue using the antibody at a dilution of 1:100. The procedure involved dewaxing and hydration, followed by antigen retrieval under high pressure in citrate buffer (pH 6.0). The tissue section was then blocked with 10% normal goat serum for 30 minutes at room temperature. The primary antibody, prepared in 1% BSA, was incubated overnight at 4°C. Detection was carried out with a goat anti-mouse IgG polymer conjugated to horseradish peroxidase (HRP), and visualization was achieved using 0.05% DAB staining.