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Mouse Anti-BCL2 mAb (Unconjugated, IgG1) [REK5-K127]

Cat. No.: REK5-K127
Type: Monoclonal Antibody
Size: 100 µL
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REK5-K127 data.
REK5-K127 data.

WB using the antibody (1:1000) detected a specific band at 26 kDa in HeLa, THP-1, NIH/3T3, and MCF-7 whole cell lysates;secondary antibody was goat anti-mouse IgG (1:20,000), with 5 min exposure.

Key Details
Type Monoclonal Antibody
Target BCL2
Species Reactivity Human;Mouse
Application ELISA;WB;Flow Cyt
Conjugation/Format Unconjugated
Host Mouse
Immunogen Recombinant human apoptosis regulator Bcl-2 protein fragment (amino acids 2-211).
Isotype IgG1
Properties
Form Liquid
Concentration Differences may occur among batches. Contact us to verify.
Dilutions WB: 1:1000-1:5000, Flow Cyt: 1:50-1:200
Purification Purified to>95% purity using Protein G affinity chromatography
Storage Buffer 0.01M PBS+50% Glycerol+0.03% Proclin 300, pH 7.4.
Storage/Stability Store immediately at -20°C or -80°C upon arrival. Repeated freeze-thaw cycles should be avoided.
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Target Aliases B-cell lymphoma 2;Bcl-2;BCL2A1
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